EAG and behavioral responses of Chilo suppressalis females to plant volatiles from Vetiveria zizanioides
Author of the article:WANG Guang-Li** FU Xiao-Gang** HAN Xiao-Xia ZHANG Yu-Yao WEI Hong-Yi**
Author's Workplace:College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
Key Words:Chilo suppressalis, female moths, vetiver volatiles, electroantennography, orientational behavior
Abstract:
[Objectives] To provide a basis for developing botanical attractants for the female rice stem borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae). [Methods] The behavioral responses of C. suppressalis females to 16 plant volatiles substances from vetiver, Vetiveria zizanioiaes, were studied using an electroantennograph (EAG) and wind tunnel techniques. [Results] All 16 substances induced EAG responses in female moths. Responses to oleic acid and cholesterol at concentrations of 0.1,1.0 and 10.0 μg/μL increased with increasing concentration, but decreased for citral, bornan2one and palmitic acid. The EAG value of C. suppressalis females to vetiver extracts at a concentration of 1.0 μg/μL was 426.11×10-3 Mv, significantly higher than that recorded for other standard compounds (P<0.05). C. suppressalis females could be attracted by volatile compounds including cholesterol, citral, n-pentadecanoic acid, myristic acid, glycerol 1-palmitate and diisooctyl phthalate in wind tunnel assays, and different ratios of these compounds could be ranked in order of decreasing attractiveness to female moths as follows, cholesterol:citral:myristic acid=33:24:11, diisooctyl phthalate:citral:glycerol 1-palmitate=130:48:1, cholesterol: citral:myristic acid: glycerol 1-palmitate=66:48:22:1, citralglycerol 1-palmitate=48:1. [Conclusion] Vetiver volatile compounds to which C. suppressalis females displayed electrophysiological and behavioral responses have potential as botanical attractants for these pests.