Effects of temperature on the expression patterns of OcsHSP genes in Oxya chinensis

Author of the article:SHI Xue-Kai1, 2, 4** KOU Li-Hua1, 4** ZHANG Yu-Ping3 MA En-Bo1, 4ZHANG Jian-Zhen1, 4 WU Hai-Hua1

Author's Workplace：1. Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; 2. College of Life Science, Shanxi University, Taiyuan 030006, China; 3. Biology Department, Taiyuan Normal University, Taiyuan 030619, China; 4. Shanxi Key Laboratory of Integrated Pest Management in Agriculture, Taiyuan 030006, China

Key Words：Oxya chinensis, small heat shock protein, temperature, real-time quantitative PCR

Abstract：

[Objectives]  Small heat shock proteins (sHSPs) can be induced by environmental stress. Different temperatures can induce the expression of sHSPs genes in Oxya chinensis. [Methods]  The effect of temperature on the mRNA expression patterns of six OcsHSP genes in fifth-instar nymphs of O. chinensis was analyzed using Real-time quantitative PCR. [Results]  There was no significant difference in the expression levels of OcHSP19.1, OcHSP19.8, OcHSP20.4 and OcHSP20.7 at 27, 32 and 37℃, but expression levels significantly increased at 42℃, especially after 2 h at 42℃. There was no significant difference in the expression levels of OcHSP21.1 and OcHSP23.8 at different temperatures. Multiple factor analysis of variance indicated that there was a significant interaction between temperature and gene. [Conclusion]  Temperature can affect the expression of sHSPs genes in O. chinensis but different genes respond differently to temperature.