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Issue:ISSN 2095-1353
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Your Position :Home->Past Journals Catalog->2017年54 No.3

Molecular cloning, sequence analysis and expression of the chitin synthase 2 gene in the oriental fruit moth Grapholitha molesta (Busck)
Author of the article:YANG Jing1** YU Qin1** GAO Yue1 LIU Zhong-Fang1 SHI Gao-Chuan2 ZHANG Peng-Jiu1 FAN Ren-Jun1**
Author's Workplace:1. Institute of Plant Protection, Shanxi Academy of Agricultural Science, Key Laboratory of Integrated Pest Management in Agriculture (IPMA), Taiyuan 030031, China; 2. Cotton Research Institute, Shanxi Academy of Agricultural Science, Yuncheng 044000, China
Key Words:Grapholitha molesta, chitin synthase, expression profile, real-time quantitative PCR
Abstract:

[Objectives]  To analyze the mRNA expression characteristics of the oriental fruit moth, Grapholitha molesta (Busck) chitin synthase 2 gene (GmCHS2) and provide a theoretical basis for studying its physiological function and developing new pest control methods. [Methods]  The cDNA of GmCHS2 (GenBank accession number: KY242360) was obtained based on the transcriptome dataset and rapid amplification cDNA end(RACE)technology. A phylogenetic tree was constructed based on the homologous sequences of other insects. Relative expression levels of the GmCHS2 gene were detected in different development stages and tissues using real-time quantitative PCR. [Results]  GmCHS2 cDNA was4 991bp in length, including a 267 bp 5¢ terminal UTR and a 170 bp 3¢ terminal UTR, encodes 1517 amino acids and contains 14 transmembrane helices. A phylogenetic tree indicates that GmCHS2 belongs to the chitin synthase 2 gene family. Analysis of GmCHS2 expression in different development stages revealed that the highest expression levels occurred in prepupae and adults. Analysis of the expression of GmCHS2 in different tissues indicates that it was highly expressed in the foregut and midgut, followed by the hindgut, and only slightly expressed, or not expressed, in other tissues. [Conclusion]  GmCHS2 may play an important role in the growth and development of G. molesta.

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