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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2017年54 No.3

Metagenome analysis of bacterial diversity in cricket intestines
Author of the article:ZHANG Ke1** LIU Wu-Xian2 ZHENG Xin-Hua1 LIU Bing-Xu3
Author's Workplace:1. Pingdingshan University, Pingdingshan 467000, China; 2. Animal Health Supervision Institute of Zhengzhou City Henan Province, Zhengzhou 450000, China; 3. Zhengzhou Zoon, Zhengzhou 450000, China
Key Words:Teleogryllus, MtDNA-16S rRNA, intestine bacteria, bacterial diversity, 16S rRNA
Abstract:

  [Objectives]  To clarify bacterial diversity in the anterior midgut and posterior intestine of Crickets. [Methods]  Molecular identification of crickets was carried out based on the cricket 16S rRNA sequence. Total DNA from the anterior midgut and posterior intestine was then isolated and bacterial 16S rRNA v3-v4 sequenced by Illumina Miseq. Finally, the bacterial community composition and diversity in anterior midgut and posterior intestine was analyzed. [Results]  Analysis of MtDNA-16S rRNA showed that all crickets belonged to the genus Teleogryllus, with the provisional species name Teleogryllus spp. from Pingdingshan. A total of 21 799 reads clustered into 875 OUTs were obtained from the anterior midgut, and 16 515 reads clustered into 2 155 OUTs from the posterior intestine.  Proteobacteria, Firmicutes and Bacteroidetes accounted for 99% of the bacteria identified in both the anterior midgut and posterior intestine. At the genus level, Lactococcus (33.47%), Pantoea (21.50%), Enterobacter (15.47%), Wolbachia (9.47%)and Weissella (5.34%) were the most abundant of 232 genera identified in the anterior midgut, and unclassified Ruminococcaceae (30.07%), Parabacteroides (10.93%), Incertae sedis (9.74%), Alistipes (5.86%), Bacteroides (5.50%), Dysgonomonas (4.91%) were the most abundant of 152 genera identified in the posterior intestine. [Conclusion]  MtDNA-16S rRNA is an effective tool for Teleogryllus molecular identification. Cricket intestines are rich in microbial resources and may have the potential for the development of such resources.

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