[Objectives]  To explore the distribution and correlation of Agrilus planipennis Fairmaire larvae and their natural enemies on the host tree. [Methods]  the spatial population niche of A. planipennis larvae and their natural enemies were studied on Fraxinus velutina in Tianjin and Beijing Cites, as well as on F. mandshurica in Liaoning Province. [Results]  The results showed that the number of A. planipennis larvae and its natural enemies decreased gradually with the height of host trees, but the vertical distribution ranges of A. planipennis larvae and its natural enemies were different between different host trees, F. mandshurica and F. velutina. Distribution scope of A. planipennis larvae and its natural enemies on F. velutina was wider than that on F. mandshurica. The synchrony of varied natural enemies to A. planipennis larvae and their utilization on space and nutrition were also different. In Tianjin area, both parasitoid Spathius agrili Yang (Hymenoptera: Braconidae) and predator woodpeckers (Piciformes: Picidae) had strong synchrony to A. planipennis larvae and thus showed intensive competition for space and host resources. Woodpeckers revealed the strongest synchrony to its prey in Beijing. While the competition among pathogen, Metapelma sp. (Hymenoptera: Eupelmidae) and S. agrili was intense. In Liaoning, distribution scope of A. planipennis larvae and its natural enemies was narrower than that in Beijing and Tianjin, so the synchrony of natural enemies to host larvae were not such apparent. But competitions for space and host resources were also tense among different natural enemies. [Conclusion]  These findings provide supports for the EAB management and natural enemies’ conservation and utilization.