Analysis of mitochondrial cytochrome oxidase Ⅰgene and genetic variation of Rhyzopertha dominica population
Author of the article:SONG Xu-Hong1, 2** WANG Ping-Ping1, 3 ZHANG Hong-Yu1***
Author's Workplace：1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Chongqing Academy of Chinese Material Medica, Chongqing 400065, China; 3. Wuhuan Polytechnic University, Wuhan 430023, China
Key Words：Rhyzopertha dominica population, mtDNA, cytochrome oxidase Ⅰ, genetic differentiation
Abstract：
[Objectives] To depth understanding the genetic structure by comparing the mtDNA-COⅠ gene fragment of different populations of Rhizopertha dominica (Fabricius). [Methods] The mtCOⅠ gene partial sequences (691 bp) of 100 individual from 15 R. dominica populations were amplified and sequenced. [Results] The molecular evolution characteristic of COⅠgene in different individuals was discussed. The results indicate that average A + T content of COⅠgene in R. dominica is 63.2%, which shows strong A + T bias. The A+ T content was 85.2%, 53.3% and 57.0% respectively in the three points. A + T content of this bias showed stronger in the first than that of the second and third points. A, T, C, G content of 33.2%, 32%, 18.7% and 16.2%, respectively. Twenty-six nucleotide sites were substituted and twenty-five heliotypes were identified in all sequences with seven shared. [Conclusion] The Nm value was 1.35 showed that there was certain gene flow among R. dominica population. AMOVA analysis of genetic structure indicated that the genetic variation of R. dominica mainly occurred among individuals.