Determination of the complete mitochondrial genome of Thyestilla gebleri and comparative analysis of the mitochondrial genome in Cerambycidae
Author of the article:YANG Jin1** CAO Li-Jun2 GENG Yong-Xin1 WEI Dan-Feng1 CHEN Min1***
Author's Workplace：1. The Key Laboratory of Silviculture and Forest Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China; 2. Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Key Words：Thyestilla gebleri, Cerambycidae, mitochondrial genome, phylogeny
Abstract：
[Objectives] Analysis of mitochondrial genome is an important way to reveal the phylogenic relationship in insects. The aim of this study was to determinate the mitochondrial genome of Thyestilla gebleri and comparatively analyze the characteristics of mitochondrial genome in Cerambycidae. We also investigated the phylogenetic position of T. gebleri and the phylogenetic relationship among species in Cerambycidae based on mitochondrial genomes. [Methods] The mitochondrial
genome of T. gebleri was determined using primer walking method. The annotation of mitochondrial genes of T. gebleri were based on the GenBank data of mitochondrial genome in Cerambycidae. The secondary structure of transport RNA (tRNA) was predicted by online tRNAscan-SE Search Server. After re-annotating the mitochondrial genome of 16 species available in GenBank, we conducted comparative analysis of the mitochondrial genomic features for Cerambycidae. A phylogenetic tree for 17 species in Cerambycidae was constructed by Maximum Likelihood method based on the nucleotide sequences of 11 protein-coding genes. [Results] The total length of mitochondrial genome of T. gebleri was 15 505 bp with an A+T content of 74.07%. It contained 13 protein coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 tRNA genes and A 872 bp-long control region, and no gene rearrangement was found. The results revealed the translocation of trn P (one of a tRNA gene) in Vesperus conicicollis. The secondary structure of tRNA genes had the typical clover structure except the absent of “D” arm in trnS1(AGN). The start codon of protein-coding genes were typical ATN (ATA, ATT, ATC, ATG) except that several special start codon (TTG, AAC, AAT, GTG) existed in Nad1, COI, ATP8 in some species. The termination codon of all the protein coding genes was common TAR (TAA, TAG), T or TA. In the phylogenetic analysis, 6 subfamilies were clustered into separate branches, and T. gebleri was clustered together with Batocera lineolata. [Conclusion] T. gebleri has similar mitochondrial genome to those previously reported longicorn beetles. Except that several tRNA genes are rearranged, the mitochondrial genes in Cerambycidae are relatively stable. Phylogenetic reconstruction supported the monophyly of the six subfamilies and presented that T. gebleri was genetically close to B. lineolata in this study.