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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2017年54 No.6

Effects of cold acclimation on the supercooling point and major cold hardiness chemicals of overwintering Dendrolimus tabulaeformis larvae
Author of the article:SHAO Yu-Ying1** FENG Yu-Qian2 TIAN Bin1 ZONG Shi-Xiang1***
Author's Workplace:1. Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China; 2. Laboratory of Forest Pathogen Integrated Biology Science, Research Institute of Forestry New Technology, Chinese Academy of Forestry, Beijing 100091, China
Key Words:Dendrolimus tabulaeformis, overwintering periods, larvae, cold acclimation, supercooling point, cold hardiness substances

 [Objectives]  To explore the effects of cold acclimation on the cold hardiness of overwintering Dendrolimus tabulaeformis larvae. [Methods]  Changes in supercooling point and major cold hardiness chemicals were measured. A thermocouple was used to measure supercooling points. Moisture, lipid, glycogen and small molecule sugar and alcohol content of larvae were determined using the delta rule, the chloroform-methanol method, phenol-sulfuric acid method and capillary gas chromatography, respectively. [Results]  The moisture content, supercooling point and lipid content of D. tabulaeformis larvae acclimated to within 5℃ below ambient temperature declined significantly, but increased in larvae acclimated to within 10℃ below ambient temperature. Glycogen content significantly increased in September, but decreased slightly in January and March after cold acclimation. Changes in small molecular sugar and alcohol content were not significant. Trehalose content slightly decreased and glycerol, glucose and galactose decreased in larvae acclimated to within 5℃ below ambient temperature, but increased when larvae were acclimated to temperatures 10℃ below ambient temperature. [Conclusion]  The observed changes in moisture, lipid, glycogen content and small molecular sugar and alcohols suggest that cold acclimation can increase the cold hardiness of D. tabulaeformis. The supercooling ability of D. tabulaeformis appears to be affected by both temperature and the time of cold acclimation, which suggests that there are optimal acclimation conditions that can maximize cold hardiness. These results provide a theoretical basis for revealing the cold hardiness mechanism and potential distribution of D. tabulaeformis.

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