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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
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Your Position :Home->Past Journals Catalog->2017年54 No.6

The application of a new fishing apparatus in obtaining serial ultrathin sections of insect antennae
Author of the article:RONG Nian-Hang1** FU Bing-Xian2*** ZHU Zeng-Rong3 HONG Jian1
Author's Workplace:1. Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China; 2. School of Agricultural and Food Science, Zhejiang Agriculture and Forest University, Linan 311300, China; 3. Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China
Key Words: exoskeleton, sensilla, peripheral innervations, transmission electron microscope, slot grids
Abstract:

[Objectives]  Transmission electron microscopy is playing a more and more important role in biological research, including tracking extended structures such as neural networks and three dimensional reconstruction. Consequently, the demand for continuous sections of biological tissues for transmission electron microscopy has greatly increased. However, limitations of the current fishing method make the acquisition of continuous sections difficult, especially when samples are comprised of dense insect exoskeleton, such as continuous sections of the antennae. Insufficient penetration of the agent usually causes poor polymerization, which undoubtedly increases the difficulty of collecting intact serial sections. To solve these problems, we developed a kind of combined device for collecting sections with slot grids. [Methods]  A hybrid device comprised of a special fishing loop and a lofting platform was developed and its ability to fish for serial sections and place them onto slot grids was evaluated. [Results]  No partial detachment and folding occurred in the serial sections obtained by this combined device; specimen sections neither separated completely, nor slid to the side of the resin section. Sample sections were intact without any damage or wrinkles, and serial sections clung to the slot area in a line. [Conclusion]  The new device is an improvement on previous methods of obtaining continuous sections of insect antennae for electron microscopy. 

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