[Objectives]  To assess geometric morphometric analysis of wing shape as a means of accurately identifying three biting midges of the genus Culicoides Latreille, C. punctatus, C. pulicaris and C. newsteadi. [Methods]  Various geometric methods were used to analyze wing shape variation, including landmarks, principal component analysis, canonical variate analysis, thin-plate splines and cluster analysis. [Results]  Significant differences in wing shape and venation were apparent among the three species, the main differences were in the radio-median cross-vein, radial cell, and basal and distal portions of the wing. C. punctatus and C. newsteadi were most similar in wing shape and venation. [Conclusion]  Geometric morphometrics is a useful tool for identifying interspecific relationships within the Ceratopogonidae.