An efficient dsRNA production method based on engineering bacteria for targeted insect genes
Author of the article:MA Zhong-Zheng** YAN Shuo*** SHEN Jie
Author's Workplace:Department of Entomology and MOA Key Laboratory for Monitory and Green Control of Crop Pest, China Agricultural University, Beijing 100193, China
Key Words: RNA interference; dsRNA production; L4440; HT115 (DE3)
Abstract:
xogenous
or endogenous double-stranded RNA (dsRNA) can be used to apply RNA interference
(RNAi) to targeted insect genes. There has recently been progress in the
utilization of RNAi technology to control agricultural pests. The high cost of
dsRNA synthesis is the main obstacle to the application of RNAi in the field.
We constructed a cheap and efficient dsRNA production method using the L4440
and HT115 (DE3) bacterial strains that can significantly reduce the cost of
dsRNA production compared to commercial kits. Our method has the potential for
large scale assays of insect gene function and for the field application of
RNAi reagents, which may promote the practice and development of RNAi-based
pest control.