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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2019年56 No.2

An efficient dsRNA production method based on engineering bacteria for targeted insect genes
Author of the article:MA Zhong-Zheng** YAN Shuo*** SHEN Jie
Author's Workplace:Department of Entomology and MOA Key Laboratory for Monitory and Green Control of Crop Pest, China Agricultural University, Beijing 100193, China
Key Words: RNA interference; dsRNA production; L4440; HT115 (DE3)

xogenous or endogenous double-stranded RNA (dsRNA) can be used to apply RNA interference (RNAi) to targeted insect genes. There has recently been progress in the utilization of RNAi technology to control agricultural pests. The high cost of dsRNA synthesis is the main obstacle to the application of RNAi in the field. We constructed a cheap and efficient dsRNA production method using the L4440 and HT115 (DE3) bacterial strains that can significantly reduce the cost of dsRNA production compared to commercial kits. Our method has the potential for large scale assays of insect gene function and for the field application of RNAi reagents, which may promote the practice and development of RNAi-based pest control.

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