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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
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Your Position :Home->Past Journals Catalog->2019年56 No.2

Identification and expression of the calcium-binding protein in MED Bemisia tabaci (Gennadius)
Author of the article:LI Shi-Xiang1, 2** PENG Zheng-Ke2 LI Chuan-Ren1 SHI Cai-Hua1, 2 WANG Shao-Li2 XIE Wen2 XU Bao-
Author's Workplace:1. College of Agriculture, Yangtze University, Jingzhou 434025, China; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Key Words:Bemisia tabaci; calcium-binding protein; gene clone; expressing analysis
Abstract: 

[Objectives]  To clarify the temporal and spatial expression of calcium-binding protein in MED (Mediterranean) Bemisia tabaci after feeding on different hosts. A component of the calcium signaling pathway, the main function of the calcium-binding protein is to bind to calcium ions, regulating cell signaling and cell life cycle processes. Calcium-binding proteins can reduce the calcium ion concentration by binding to calcium ions in plants, thereby maintaining a continuous intake by insects. [Methods]  The cDNA sequence of the B. tabaci calcium binding protein was amplified using the reverse transcription polymerase chain reaction (RT-PCR) and gene cloning, and its expression in all developmental tissues and stages, hosts, and after feeding on different plants, analyzed using Real-time quantitative PCR. [Results]  Bioinformatics analysis indicates that the open reading frame of the B. tabaci calcium-binding protein gene is 669 bp, encoding a protein comprised of 222 amino acids. The N-terminus contains a signal peptide sequence of 22 AA and the C-terminal has a Ca2+ binding site. Phylogenetic analysis shows that the B. tabaci calcium-binding protein is closely related to that of other Hemipteran insects, and belongs to the same branch. The results of real-time PCR show that the expression of the calcium-binding protein in the head of B. tabaci was significantly higher than in the thorax, abdomen, leg and wing. The calcium-binding protein was expressed in every developmental stage of B. tabaci; expression was highest in fourth-instar nymphs and lowest in eggs. Expression levels in first, second, third and fourth instars, and in adults, were 4.38 times, 5.47 times, 16.76 times and 5.03 times, higher than in the egg stage, respectively. Feeding B. tabaci on pepper, tomato and cotton had no significant effect on the expression of the calcium-binding protein. The expression of the calcium-binding protein in adults that were allowed to fed 1 h after starvation was significantly (2.12 times) higher than in those fed 0 h after starvation. [Conclusion]  The calcium-binding protein gene of B. tabaci can be successfully cloned and its expression pattern analyzed. The results of this study provide a foundation for further study of the function of this gene in the development of B. tabaci.

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