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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2019年56 No.2

Genetic diversity and genetic differentiation of Calliptamus barbarus in Sino-Kazakh border areas
Author of the article:MAI Ji-Wei1** XU Ye2 SHI Yi1 WANG Tian-Xin1 ROMAN Jashenko3 ZHAO Wei1 WANG Han2***
Author's Workplace:1. School of Life Sciences, Lanzhou University, Lanzhou 730000, China; 2. International Research Center of Cross-border Pest Management in Central Asia, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China;3. Al-Farabi Kazakh National University, Almaty 050038, Kazakhstan
Key Words:Calliptamus barbarus; mitochondrial DNA; COⅠ gene; geographic population; gene flow
Abstract: 

Abstract  [Objectives]  To investigate the genetic diversity of Calliptamus barbarus in the border area between China and Kazakhstan, and reveal the molecular mechanism underlying serious regional outbreaks of this pest. [Methods]  Specimens of C. barbarus were collected from 5 different geographic populations (ALT, TC, BL, YL and Kazakhstan) on the border between China and Kazakhstan. A total of 100 full-length sequences of the mitochondrial cytochrome oxidase subunit I (CO) gene (1 540 bp) were obtained, sequenced, and compared, using DnaSP 5.0 and Arlequin 3.5 software. [Results]  About 122 mutations were found, including 69 haplotypes, one of which was shared by all 4 populations. The genetic distance between populations ranged from 0.001 to 0.086 and the total haplotype diversity index (Hd) was 0.987. The diversity of haplotypes among different geographic populations ranged from 0.973 to 0.995. The total nucleotide diversity (Pi), total genetic differentiation coefficient (Gst), total fixed coefficient (Fst), and total gene flow (Nm) were 0.008 4, 0.004 5, 0.014 2 and 4.61, respectively. Neutrality test resultsTajima′s D=-1.553 3P >0.05Fu’s Fs=-3.732 4P >0.05 indicated that C. barbarus populations on the border between China and Kazakhstan have not recently expanded. The information obtained from the haplotype network and a neighbour-joining phylogenetic tree were consistent, and the differentiation of the BL population from other populations was obvious. The genetic difference between populations (1.44%) is much less than the genetic variation within populations, indicating that geographic distance has no obvious effect on the genetic distance between different populations. No obvious geographic structure is apparent in a phylogenetic tree and a haplotype network based on haplotypes of the different geographic populations. [Conclusion] There is frequent gene exchange between different geographic populations of C. barbarus in the border area between China and Kazakhstan and genetic differentiation of this species in this region is consequently low.

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