Changes in the fat, carbohydrate and protein content of Aspongopus chinensis during diapause
Author of the article:WU YouFang；ZHOU WenZhen；ZHAO Shuai；JIN DaoChao；GUO JianJun
Author's Workplace：Institute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guiyang 550025, China；Institute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guiyang 550025, China；Institute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guiyang 550025, China；Institute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guiyang 550025, China
Key Words：Aspongopus chinensis; diapause; fat; carbohydrates; protein
Abstract：[Objectives] To investigate changes in the fat, carbohydrate and protein content of Aspongopus chinensis during diapause. [Methods] The fat, carbohydrate and protein content of A. chinensis were measured using Soxhlet extraction, anthrone colorimetric measurement and a BCA protein assay kit, respectively, from October (the beginning of diapause) to May (the end of diapause). [Results] The fat content of A. chinensis decreased gradually during diapause to a minimum in April (19.51%), which was significantly lower (P<0.05) to that in the early stage of diapause (October) (40.55%). The carbohydrate content of A. chinensis increased in the early stage of diapause until November, then decreased until April, a significant (P<0.05) increase compared to when diapause terminated in May. The protein content of A. chinensis slowly increased from the early stage of diapause (October), began to decline in March, and reached its lowest level in May, which was significantly lower than that during diapause (P<0.05). [Conclusion] The fat, carbohydrate and protein content of A. chinensis change to different degrees during diapause and these changes are major physiological characteristics of the diapause process.