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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2019年56 No.5

Analysis of antennal transcriptome and olfaction-related genes of adult Batocera horsfieldi (Hope)
Author of the article:HU JiaMeng;XU DanPing;ZHUO ZhiHang;YANG Wei;YANG Hua;ZHENG YiRan
Author's Workplace:1. College of Forestry, Hainan University, Haikou 570228, China; 2. College of Food Science, Sichuan Agricultural University, Yaan 625014, China; 3. College of Forestry, Sichuan Agricultural University, Chengdu 611130, China
Key Words: Batocera horsfieldi (Hope); antennal transcriptome; olfaction-related genes; genome annotation; high-throughput sequencing
Abstract:[Objectives]  To obtain genetic data on, and establish an antenna transcriptome database for, the adult white-striped longhorn beetle, Batocera horsfieldi (Hope). [Methods]  The antenna transcriptome of adult B. horsfieldi was sequenced using an Illumina HiSeq 4000 platform and analyzed with bioinformatic software. [Results]  The antennal transcriptome database of adult B. horsfieldi contained 137 485 transcripts and 69 214 Unigenes. The mean number of transcripts was 1 142 bp, and the mean number of Unigenes was 1 983 bp. Of the Unigenes found, 41 636 were annotated in the NR database, 14 895 were annotated in the NT database, 19 287 were annotated in the KO database, 33 442 were annotated in the SwissProt database, 34 687 were annotated in the PFAM database, 35 321 were annotated in the GO database, and    20 582 were annotated in the KOG database. The results of NR comment showed that 72.0% of B. horsfieldi Unigenes were similar to those of Tribolium castaneum and Dendroctonus ponderosae. Transcriptome gene function classification indicated five main functional classifications annotated in the GO database; these were cellular process, metabolic process, single-organism process, binding and catalytic activity, each classification containing 20 912, 19 086, 17 202, 21 477 and   15 823 unigenes, respectively. 20 585 unigenes were annotated in 26 groups of the KOG database, 1 977 of which were involved in post-translational modification, protein turnover and chaperoning. Only 3 285 unigenes were involved in general function prediction, and 3 053 in signal transduction mechanisms, together comprising 40.39% of the total. 19 287 unigenes were annotated in 5 functional classifications in the KEGG database; cellular process (6 793), environmental information processing (6 255), genetic information processing (3 038), metabolism (3 852) and organismal system (3 508). By further screening and identification, 161 olfaction-related genes, including 96 odorant binding protein (OBP) genes, 34 chemosensory protein (CSP) genes and 31 odorant receptor (OR) genes, were identified. [Conclusion]  The transcriptome database of the antenna of adult B. horsfieldi was successfully obtained. The results provide a foundation for the further study of gene function and the molecular mechanisms responsible for olfactory reception in B. horsfieldi.
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