[Objectives]  The whitefly, Trialeurodes ricini is an important crop pest in China. Wolbachia is the most abundant intracellular bacterial endosymbiont widely distributed in arthropods, including T. ricini and the parasitic wasp Encarsia formosa. Wolbachia has an important influence on the reproduction and development of insects so knowing it’s distribution in T. ricini and E. formosa can lay a foundation both for the in-depth study of Wolbachia, and novel ideas for the biocontrol of whiteflies. [Methods]  The distribution of Wolbachia in indoor populations of T. ricini and E. formosa were detected by PCR. Specific primers of the Wolbachia wsp gene within groups A and B were designed. The amplified genes were sequenced and a phylogenetic tree was constructed. [Results]  Only group A was detected in the T. ricini population with an infection rate of 40%. Phylogenetic analysis indicated that this group of Wolbachia, together with the Wolbachia reported in parasitic wasps, belongs to the Dro subgroup of group A. The Wolbachia detected in the E. formosa population belonged to group B. These two strains of Wolbachia did not horizontally transfer between the T. ricini and E. formosa. [Conclusion]  The infection rate of Wolbachia in an indoor breeding population of the T. ricini was only 40%, which indicates that although Wolbachia can be inherited in T. ricini, its ability to spread horizontally through the population is weak. We conclude that Wolbachia doesn’t spread horizontally between T. ricini and E. formosa populations over a short time period.