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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2020年57 No.6

Evaluating insecticide bioassay methods using the Spodoptera frugiperda cell line Sf 9
Author of the article:LI Chun-Na ZHANG Wei-Qi REN Dou-Dou DONG Zhao-Ke ZHANG Zhi-Yong
Author's Workplace:Beijing Agricultural College, Beijing 102206, China; Qingdao Agricultural University, Qingdao 266109, China
Key Words:Sf 9 cells; Plutella xylostella; insecticide; bioassay; LC50
[Objectives]  To meet the need for rapid bioassay methods for pesticide development and pest resistance monitoring, we compared the results obtained from bioassays using insect cells vs whole insects. [Methods]  The virulence of different pesticides to the Spodoptera frugiperda ovary cell line (Sf 9) and sensitive strains of Plutella xylostella was examined, and the correlation between the inhibition of the cell proliferation rate and insect mortality compared. [Results]  Both bioassay systems ranked the virulence of the three different types of insecticides as avermectin > imidacloprid > beta- cypermethrin, and the virulence of the five insecticides in the same category (organophosphorus) as > dichlorvos > phoxim > propionate > trichlorfon. Both bioassay systems also ranked the virulence of three formulations of the same insecticide (5% avermectin) as micro-emulsionn > emulsifiable concentrate > emulsion. A linear regression model of the relationship between an 8 h cell bioassay and 48h insect bioassay was constructed. [Conclusion]  The results of an 8 h cell bioassay were consistent with those of a 48 h insect bioassay. Cell bioassays provide a relatively quick way of testing new pesticides.
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