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Your Position :Home->Past Journals Catalog->2021年58 No.2

DNA barcoding of Anagrus dmitrievi (Hymenoptera, Mymaridae)
Author of the article:Nuriye·Muhetaier;CHEN Guang-Hui;PENG Jie;GAO Bin;ZHANG Xiao-Dong;XI Ou-Yan;Julidezi·Aishan
Author's Workplace:College of Life Science and Technology, Xinjiang University, Urumqi 830046, China; Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China; Kashi Customs Technology Center, Kashi 844000, China
Key Words:Anagrus dmitrievi; non-damage DNA extraction; DNA barcoding; COⅠ genes; ITS2 genes
Abstract:
[Objectives]  To provide a convenient and efficient way of identifying Anagrus dmitrievi Triapitsyn & Hu, a small parasitic wasp with potential as a biological control for the corn pest Zyginidia eremita Zachvatkin (Hemiptera, Cicadellidae, Typhlocybinae), that is difficult to identify on the basis of morphological features. [Methods]  Eggs of Z. eremita were collected and hatched in a laboratory to obtain adult A. dmitrievi which were then identified according to morphological characters such as body color, antennae and wings. Genomic DNA was extracted from the adult wasps using a non-damaging DNA extraction method, and DNA barcoding tests performed on the CO and ITS2 genes after PCR amplification and sequencing. [Results]  Multiple sequence alignments, genetic distance and phylogenetic analyses indicate that although A. dmitrievi CO and ITS2 are highly homologous to those of other Anagrus species listed in the NCBI databases, there are some clearly distinguishable differences. [Conclusion]  CO and ITS2 gene sequences were obtained from male and female A. dmitrievi are sufficiently distinctive to provide a foundation for the development of a molecular rapid identification system for this small, parasitic wasp.
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