[Objectives]  Based on the biological missile concept, the use of pathogen-carrying parasitic insects is potentially a new way of preventing and controlling both Monochamus alternatus and pine wilt disease. [Methods]  We measured the virulence of conidial powder and spore suspensions of four pathogenic fungal strains carried by Scleroderma sichuanensis, and the initial and residual pathogen load of each strain. We then analyzed the effects of each pathogenic strain and pathogen load on S. sichuanensis, and the shedding of pathogen spores on the body surface this species. [Results]  The mortality rate of females carrying conidial powder was higher than that of those infected with spore suspension, but the pathogen load of conidial powder was much higher than that of spore suspension. For suspensions, both the cumulative mortality and pathogen load of S. sichuanensis increased with increasing concentration. The virulence and the mortality rate induced by Beauveria bassiana was higher than that of Metarhizium anisopliae, but the pathogen load of B. bassiana was higher than that of M. anisopliae. Irrespective of dosage or strain, most spores on the body surface of S. sichuanensis fell off after the first day after which the rate of spore loss continued more slowly. [Conclusion]  S. sichuanensis is an effective vector of fungal pathogens of M. alternatus, particularly B. bassiana.