Analysis of the olfactory genes of Corcyra cephalonica (Lepidoptera: Pyralidae)
Author of the article:LI Peng-Yan ZHANG Qiu-Ting HUANG Zheng-Yu HUANG Hua CHEN Wei-Ping LIAO Yong-Lin
Author's Workplace:Guangdong Academy of Agricultural Sciences, Plant Protection Research Institute, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China; National Engineering Laboratory of Deep Processing of Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Key Words:Corcyra cephalonica; transcriptome sequencing; olfactory genes; function annotation
Abstract:
[Objectives] To obtain olfactory
gene information and establish a transcriptome database for the adult rice
moth, Corcyra cephalonica. [Methods] The C.
cephalonica transcriptome was sequenced on the Illumina HiSeq
platform, the original data were assembled and analyzed using the Trinity
software package and Blast was used to annotate unigenes in 7 databases. The
expression of pheromone binding proteins (PBPs) and general odorant binding
proteins (GOBPs) in different adult moth tissues was then detected using PCR. [Results] A total of 58 534 unigenes
were obtained, 25 879 of which were annotated with an N50 value of 2 212 bp and
mean length of 1 251 bp. C.
cephalonica unigenes had highest similarity (28.2%) to those of Amyelois transitella; 20 odorant binding
proteins (OBPs) genes, 18 chemosensory proteins (CSPs) genes, 2 general odorant
binding protein (GOBPs) genes and 3 pheromone binding proteins
(PBPs) genes, were identified and uploaded to NCBI. PBPs and GOBPs were highly
expressed in the antennae of adult moths but expression was lower in the head,
thorax, abdomen, leg and wing. PBP3 was not expressed in the thorax, and GOBP2
was only highly expressed in the antennae and not in other tissues. [Conclusion] The results significantly increase the
available information on the olfactory genes of C. cephalonica, and provide a molecular foundation for the
study of olfactory recognition mechanisms and the development of attractants for
this pest.