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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2021年58 No.6

Analysis of the olfactory genes of Corcyra cephalonica (Lepidoptera: Pyralidae)
Author of the article:LI Peng-Yan ZHANG Qiu-Ting HUANG Zheng-Yu HUANG Hua CHEN Wei-Ping LIAO Yong-Lin
Author's Workplace:Guangdong Academy of Agricultural Sciences, Plant Protection Research Institute, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China; National Engineering Laboratory of Deep Processing of Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Key Words:Corcyra cephalonica; transcriptome sequencing; olfactory genes; function annotation
Abstract:
[Objectives]  To obtain olfactory gene information and establish a transcriptome database for the adult rice moth, Corcyra cephalonica. [Methods]  The C. cephalonica transcriptome was sequenced on the Illumina HiSeq platform, the original data were assembled and analyzed using the Trinity software package and Blast was used to annotate unigenes in 7 databases. The expression of pheromone binding proteins (PBPs) and general odorant binding proteins (GOBPs) in different adult moth tissues was then detected using PCR. [Results]  A total of 58 534 unigenes were obtained, 25 879 of which were annotated with an N50 value of 2 212 bp and mean length of 1 251 bp. C. cephalonica unigenes had highest similarity (28.2%) to those of Amyelois transitella; 20 odorant binding proteins (OBPs) genes, 18 chemosensory proteins (CSPs) genes, 2 general odorant binding protein (GOBPs) genes and 3 pheromone binding proteins (PBPs) genes, were identified and uploaded to NCBI. PBPs and GOBPs were highly expressed in the antennae of adult moths but expression was lower in the head, thorax, abdomen, leg and wing. PBP3 was not expressed in the thorax, and GOBP2 was only highly expressed in the antennae and not in other tissues. [Conclusion]  The results significantly increase the available information on the olfactory genes of C. cephalonica, and provide a molecular foundation for the study of olfactory recognition mechanisms and the development of attractants for this pest.
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