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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
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Your Position :Home->Past Journals Catalog->2021年58 No.6

Using insects and bacteria to dispose of nematode-infected pine timber
Author of the article:CHEN Hao-Nan YAN Zhen-Tian YANG Shi-Zhang LIU Yu-Sheng CHEN Bin
Author's Workplace:Chongqing Key Laboratory of Vector Insects; Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing 401331, China; Chongqing Station of Forest Pests Control and Quarantine, Chongqing 400700, China; Institute of Environmental Biology and Insect Resources, College of Plant Protection, Shandong Agriculture University, Taian 271018, China
Key Words:pine wood nematode disease; conversion treatment; Pleurotus pulmonarius; Protaetia (Liocola) brevitarsis (Lewis)
Abstract:
[Objectives]  To investigate a new, pollution-free method of disposing of pine wood infected with nematodes and provide new ideas for the effective utilization of spent mushroom substrates used in the production of edible fungi. [Methods]  The ability of the fungi Pleurotus pulmonarius and larvae of the beetle Protaetia (Liocola) brevitarsis (Lewis) to dispose of pine wood infected with nematodes was investigated. Eight strains of P. pulmonarius (X1, X2, X3, X4, X5, X6, X7, X8) and 1 strain of Pleurotus ostreatus (P) were classified using by antagonistic tests. Two kinds of culture medium were provided to these strains: 1) 81% saw dust, 15% bran, 2% lime and 2% gypsum; 2) 66% saw dust, 15% bran, 15% cottonseed hull, 2% lime, 2% gypsum. The strains of P. pulmonarius were inoculated into a culture medium made of nematode-infected pine sawdust which was cultured at 25℃ for 30-40 d after which the hyphal growth rate was calculated. P. (Liocola) brevitarsis larvae were fed broken, spent mushroom substrate under different environmental conditions and were weighed after 15 d. Larval frass and surplus spent mushroom substrates were collected, and feed intake, feed utilization rate, insect body conversion rate and approximate digestibility calculated. The seed germination index (GI) was used to test the phytotoxicity of the larval frass and spent mushroom substrate to P. pulmonarius. [Results]  The optimum temperature for P. brevitarsis larvae to feed on spent, P. pulmonarius mushroom substrate was 27 ℃ and the optimum water content was 55%-65%. 3rd instar larva were the most efficient feeders. Under optimum feeding conditions, 4.62 g of 3rd instar larvae and 80.22 g of larvae frass could be obtained from 100 g of spent P. pulmonarius substrate. The GI of spent P. pulmonarius substrates was < 50%, and there was still some phytotoxicity. The GI of larval frass was > 50% and there was no phytotoxicity. [Conclusion]  The edible fungus P. pulmonarius and larvae of the beetle P. brevitarsis larvae can be used to decompose nematode-infected pine wood.
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