[Objectives]  To determine the effect of different cold storage conditions on the cold tolerance of Aphidoletes aphidimyza (Rondani), the larvae of which are used to control aphids, in order to determine optimal conditions for the cold storage of this biological control agent. [Methods]  The supercooling point (SCP) and freezing point (FP) of all life stages of A. aphidimyza were measured using a thermocouple under different cold storage conditions. [Results]  The SCP and FP of different developmental stages of A. aphidimyza were significantly different. At 25 ℃, 2nd instar larvae had the lowest SCP (﹣26.18 ℃) and FP (﹣24.98 ℃), whereas adult males had the highest SCP (﹣22.95 ℃) and FP (﹣21.86 ℃). The SCP of pupae in each cold storage treatment increased with storage duration from 10 to 30 d and was higher than that of the control group (25 ℃). However, the SCP of refrigerated pupae for 40 days was lower than the control group. The different treatment groups within the same storage period had no effect on the SCP of pupae. The FP of pupae in the T1 treatment group (5 ℃ with 4 h at 7 ℃ daily) was lowest at 50 d and highest at 30 d. The FP of pupae in the T2 treatment group (5 ℃ with 4 h at 9 ℃ daily) was lowest at 40 d and highest at 20 d. Different treatment groups with the same storage period had no effect on the FP of pupae. [Conclusion]  2nd instar larvae is the most cold tolerant, and female adults are more cold tolerant than males. Both fluctuant and constant low temperature storage are suitable for A. aphidimyza.