[Objectives]  To investigate the mitochondrial genome of Microconema clavata (Meconematinae, Tettigoniidae, Orthoptera), and thereby enhance the Meconematinae’s mitochondrial genome database to facilitate mitochondrial genome comparisons and phylogenetic research on this group. [Methods]  We measured, assembled, annotated and analyzed, the mitochondrial genome of M. clavate, in order to determine the phylogenetic relationships of this species within the Meconematinae. We used next-generation sequencing technology to sequence the genome of M. clavate, assembled the complete mitochondrial genome sequence, then annotated and analyzed it in detail. The mitochondrial genome sequences of 12 species of the Meconematinae, including M. clavate, were selected to construct a phylogenetic tree based on differences in 13 protein coding genes and two rRNA genes. [Results]  The total length of the mitochondrial genome sequence of M. clavate was 15 618 bp, which contained 13 protein coding genes, 22 tRNA genes, two rRNA genes and one control region. The genes were arranged compactly and had the same sequence as ancestral species. The A+T of the mitochondrial genome of M. clavate accounted for 70.7% of the total bases, and there was an obvious AT bias. With the exception of ND1 which began with TTG, the remainder of the 13 protein coding genes began with ATN. All genes have TAG or TAA as the termination signal, except COX1, ND5 and ND4, which have an incomplete termination codon T. All other tRNAs have a typical clover structure except tRNA^Ser(AGN). [Conclusion] The complete mitochondrial genome sequence of M. clavate was obtained. A Bayesian phylogenetic tree indicates that M. clavata is most closely related to Xiphidiopsis gurneyi, Xizicus maculatus and Xizicus fascipes.