Abstract  [Aim]  The spillover of new pathogens, potentially as a result of the co-occurrence of viruses in wild bees and   honeybees, is a significant factor contributing to the decline in breeding bee colonies. The continuous expansion in the scale and scope of the beekeeping industry may also pose a threat to the health of wild bee pollinators in China. In this study, we conducted a survey on the infection status of bee RNA virus disease across six wild bee species in Xishuangbanna, a region of China known for its rich diversity in bee species. The data obtained from this research will enhance our understanding of RNA virus infections in wild bee species in this tropical region and aid in the protection of local pollinator species. [Methods]  Five honeybee viruses, including Black queen cell virus (BQCV), Sacbrood bee virus (SBV), Chronic bee paralysis virus (CBPV), SBV, and Israel acute paralysis virus (IAPV) were investigated in six wild pollinating bee species from Xishuangbanna, including four species of stingless bees (Tetragonula pagdeni, Lepidotrigona flavibasis, Tetragonula laeviceps, Lepidotrigona terminata), and two species of honeybee (Apis dosata, Apis florea). [Results]  Black queen cell virus (BQCV) was found to be the most prevalent virus (56.7%), followed by SBV (53.3%), CBPV (44.4%) SBV (38.9%), and IAPV (25.6%). Multiple virus co-infections were identified across all six bee species, with SBV detected and quantified in each. The results showed that the prevalence of SBV was highest in Apis florea and lowest in Apis dorsata. Amino acid sequence analysis of the Lp region from different SBV strains between the six bee hosts showed a high level of diversity compared to the original host (Apis mellifera) virus strain. The amino acid sequences from Apis florea, Lepidotrigona flavibasis, and Lepidotrigona terminata showed a high degree of similarity to the variant strain, which could be a novel sequence. [Conclusion]  This research contributes valuable insights into the distribution of bee virus hosts, driven by the continuous expansion of the breeding range of introduced honeybee species (Apis mellifera). The emergence of new pathogens in A. mellifera could pose a threat to important local pollinators.