Abstract  [Aim]  This study investigates the sublethal effects of tetrachlorantraniliprole on the predatory efficacy of Neoseiulus cucumeris (Oudemans), a known predator of citrus the yellow mite. The safety of tetrachlorantraniliprole for these predatory mites was also evaluated. [Methods]  The toxicity of tetrachlorantraniliprole on N. cucumeris was assessed under laboratory conditions using the leaf-dip method and the secondary poisoning phenomenon method. Additionally, the influence of sublethal doses of tetrachlorantraniliprole (LC₁₅ and LC₃₀, with water as a control) on the prey consumption, functional response, and predatory rate of female N. cucumeris preying on Eotetranychus kankitus (Ehara) was determined. [Results]  The LC₅₀ of tetrachlorantraniliprole on N. cucumeris, based on the leaf residue method and the secondary poisoning phenomenon methods, was 400.289 and 3.86×10⁵ µg/mL, respectively. The functional response type of female N. cucumeris to E. kankitus was not affected by exposure to sublethal concentrations of tetrachlorantraniliprole and remained at Holling-Ⅱ. However, exposure of N. cucumeris to sublethal concentrations of tetrachlorantraniliprole resulted in increased prey handling time and a decrease in the maximum daily prey consumption. Furthermore, when exposed to sublethal concentrations of tetrachlorantraniliprole, the consumption and predation rate of N. cucumeris on E. kankitus significantly decreased. [Conclusion]  The findings of this study indicate that tetrachlorantraniliprole has a negative effect on the predatory capacity of N. cucumeris. Therefore, a certain safety interval should be maintained when releasing N. cucumeris in conjunction with the use of tetrachlorantraniliprole.