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Key Words：Pamphagidae, chromosome, karyotype, Cbanding, China
Abstract：The chromosomal C-banding karyotype is useful for species identification and phylogenetic analyses. The location, size and number of C-bandings and the content of heterochromatin reflect the relationships between different genera, species and even subspecies. The chromosomal Cbanding karyotypes in the spermatogenesis of Pseudotmethis rubimarginis Li and Beybienkia songorica Tzyplenkov (Orthoptera Pamphagidae) from China were investigated. The diploid numbers of the 2 species of Pamphagidae are 19 (2n♂ ). All chromosomes are acrocentric with paracentromeric C-bands, and the mechanism of sex determination are XO(♂ ). There are some differences in the relative length of chromosomes and in the size, location and degree of dyeing of C-bands between the 2 species. This may be used to distinguish the 2 species.