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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2008年45 No.3

Identification of Rhopalosiphum maidis in key predators using COⅡ marker
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Key Words:Rhopalosiphum maidis, cytochrome oxidase subunitⅡgene, predatory natural enemy, gut content analysis, DNA marker
Abstract:One pair of sequence-characterized primer of Rhopalosiphum maidis Fitch was selected to detect the remains of R. maidis in the gut of Harmonia axyridis (Pallas) and Chrysopa pallens (Rambur) reared in laboratory and various predators collected in maize fields. The specificity tests showed the presence of the band only in R. maidis. Using this pair of primer, the halflives of DNA detectability from a single R. maidis consumed by adult H. axyridis and C. pallens were 2.814 h and 2.441 h, respectively, after feeding. The positive responses of R. maidis DNA in predators collected in corn fields, such as the larvae of Syrphus corollae Fabricius, Chrysopas, H. axyridis, Propylaea japonica (Thunberg), and adult H. axyridis and P. japonica were over 40%.
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