Identification of Rhopalosiphum maidis in key predators using COⅡ marker
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Key Words：Rhopalosiphum maidis， cytochrome oxidase subunitⅡgene， predatory natural enemy， gut content analysis， DNA marker
Abstract：One pair of sequence-characterized primer of Rhopalosiphum maidis Fitch was selected to detect the remains of R. maidis in the gut of Harmonia axyridis (Pallas) and Chrysopa pallens (Rambur) reared in laboratory and various predators collected in maize fields. The specificity tests showed the presence of the band only in R. maidis. Using this pair of primer, the halflives of DNA detectability from a single R. maidis consumed by adult H. axyridis and C. pallens were 2.814 h and 2.441 h, respectively, after feeding. The positive responses of R. maidis DNA in predators collected in corn fields, such as the larvae of Syrphus corollae Fabricius, Chrysopas, H. axyridis, Propylaea japonica (Thunberg), and adult H. axyridis and P. japonica were over 40%.