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2008年45 No.3
A comparative study on isozymes of different splash types of Harmonia axyridis
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Key Words:Harmonia axyridis, isozyme, cluster analysis, PCA(principal components analysis)
Abstract:EST, CAT, α- AMY and MDH isozymes of fifteen splash types of Harmoniaaxyridis (Pallas) were studied using vertical PAGE. The results showed that a total of 25 isozyme loci were obtained, of which 20 loci were polymorphic (PPB=80%). According to the results, the genetic similarity coefficient was 0.44 to 0.96 The average genetic distance was 0.42 The clustering dendrogram was constructed by UPGMA method. Fifteen types were divided into two major groups between which the similarity coefficient was 0.65 PCA (Principal components analysis) clarified that three splash types (H. axyridis ab.conspicua Fald., H. axyridis ab.falcata Hem.and H. axyridis ab.circumscripta Hem.) would be the major parts of genetic variation in H. axyridis(Pallas).