Histochemical localization of NADPHdiaphorase in the nervous system of tussah larva.
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Key Words:tussah Antheraea pernyi,larva,nervous system,NADPH diaphorase,histochemistry
Abstract:The distribution, number and morpha of nitric oxide synthase (NOS) positive neurons and fibers histochemically whole-mount stained for NADPH diaphorase were studied in the nervous system of tussah (Antheraea pernyi Guérin-Meneville) larva. The result indicated: five stadia of tussah larva have positive reaction of NADPH-d in the brain and all the ganglia. The positive neuron can be divided into A, B, and C types. The A type cell was located along the median of ganglion, and have more intensity reaction, the long diameter about 30~70 μm. The number of A type cell was invariableness in same ganglion of each stadium. The B type cell was located at the edge of ganglion, and reaction was more weak than A type cell, the long diameter about 8~20 μm. The number of B type cell was variableness in same ganglion of each stadium, and tends to decrease follow the development of larva. The C type cell was located at the subpharyngeal ganglion and eighth abdominal ganglion, and reaction was most strong in three type cells, the long diameter about 16~50 μm.