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Your Position :Home->Past Journals Catalog->2008年45 No.1

Abundance of microsatellites in the entire genome and EST of Tribolium castaneum
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Key Words:microsatellite, T. castaneum, genome, EST, abundance
Abstract:Microsatellites are genetic markers that have made great advances in recent years. In order to improve the studies related to genetics of Tribolium castaneum(Herbst), simple sequence repeats of the entire genome and EST in T. castaneum with 1~6 bp nucleotide motifs were analyzed. Abundance of microsatellites across the genome of T. castaneum was analyzed and compared between all of the chromosomes. The results showed that the frequency of SSRs in the EST was 1/0.87 kb, among which mononucleotide repeat motifs appeared to be the most abundant type (71.25%), while the hexanucleotide, trinucleotide, tetranucleotide, dinucleotide and pentanucleotide repeats accounted for 23.93%, 2.94%, 1.56%, 0.17% and 0.15% of the total, respectively. The frequency of SSRs in the entire genomewas 1/3.65 kb, of which hexanucleotide repeat motifs were the most abundant type (61.91%), while trinucleotide, tetranucleotide, mononucleotide, pentanucleotideand dinucleotide repeats were 14.35%, 13.75%, 4.68%, 3.6% and 1.69%, respectively. Meanwhile, the Arich and Trich repeats were predominant, whereas G-rich and C-rich repeats were rare. Further analysis demonstrated that the abundance of microsatellites in different chromosomes was to a great extent similar.
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