Abundance of microsatellites in the entire genome and EST of Tribolium castaneum
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Key Words：microsatellite, T. castaneum, genome, EST, abundance
Abstract：Microsatellites are genetic markers that have made great advances in recent years. In order to improve the studies related to genetics of Tribolium castaneum(Herbst), simple sequence repeats of the entire genome and EST in T. castaneum with 1～6 bp nucleotide motifs were analyzed. Abundance of microsatellites across the genome of T. castaneum was analyzed and compared between all of the chromosomes. The results showed that the frequency of SSRs in the EST was 1/0.87 kb, among which mononucleotide repeat motifs appeared to be the most abundant type (71.25%), while the hexanucleotide, trinucleotide, tetranucleotide, dinucleotide and pentanucleotide repeats accounted for 23.93%, 2.94%, 1.56%, 0.17% and 0.15% of the total, respectively. The frequency of SSRs in the entire genomewas 1/3.65 kb, of which hexanucleotide repeat motifs were the most abundant type (61.91%), while trinucleotide, tetranucleotide, mononucleotide, pentanucleotideand dinucleotide repeats were 14.35%, 13.75%, 4.68%, 3.6% and 1.69%, respectively. Meanwhile, the Arich and Trich repeats were predominant, whereas G-rich and C-rich repeats were rare. Further analysis demonstrated that the abundance of microsatellites in different chromosomes was to a great extent similar.