A method for tissue localization of prophenoloxidase based on colloidal gold labeled immunoelectron microscopy

Key Words：Ostrinia furnacalis, prophenoloxidase, colloidal gold, immunoelectron microscopy

Abstract：       A modified immunogoldlabeling technique based on Epon 812 embedding specimen was used to localize prophenoloxidase (PPO) in the midgut and integument tissue from the 5th instar larvae of Ostrinia furnacalis Guenée. The mouse anti-O. furnacalis PPO serum prepared in our laboratory was used as primary antibody and colloidal gold labeled goat antimouse IgG as the secondary antibody. Bis (acetato) dioxouranium and lead citrate were used in the staining system. Both the labeling specific and resolution of electron microscopy were improved.