olecular cloning and sequences analysis of molt hormone receptor 3 (HR3) cDNA from Agrotis c-nigrum and Mythimna separata
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Key Words：Agrotis c-nigrum, Mythimna separata, hormone receptor 3 (HR3), molecular ing, sequence analysis
Abstract： sect molt hormone receptor 3 (HR3) is a molt-regulating transcription factor that plays an important role in regulating insect molt. Total RNA was isolated from the prepupae of Agrotis c\|nigrum L. and Mythimna separata Walker. The cDNA sequences were cloned by RT-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence of A. c-|nigrum HR3 was 1 729 base pairs in length and contained an open reading frame of 1 533 base pairs coding for a polypeptide of 510 amino acid residues with a predicted molecular weight of 57.5 ku. The cDNA sequence of M. separate HR3 was 1 743 base pairs in length and contained an open reading frame of 1 536 base pairs coding for a polypeptide of 511 amino acid residues with a predicted molecular weight of 57.9 ku. The predicted protein of both insects had the characteristics of the nuclear receptor superfamily and shared extensive similarities with those from other insects，especially the Lepidoptera. The cDNA sequences have been deposited in GenBank with accession numbers GU188853 for A. c-nigrum HR3 and GU188854 for M. separata HR3.