A method to identify and analyze salivary enzymes of Bemisia tabaci adults
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Key Words： piercing-sucking insect, Bemisia tabaci, saliva collection, identification and analysis
Abstract： A method to identify and analyze the salivary enzymes of small piercing-sucking insects, including artificial feeding, saliva collection, enzyme identification and analysis of salivary polyphenol oxidase and peroxidase, was developed using Bemisia tabaci (Gennadius) B\|biotype adults. The result show that PPO and POD activity in the watery saliva of B. tabaci B\|biotype feeding on cabbage were respectively 1.54 and 1.65 fold higher than those feeding on tomato. This new method provides a simple intuitive and sensitive way of identifying and measuring the activity of enzymes in insect saliva. This method can also be applied to other small piercing-sucking insects, such as aphids and psyllids.