20Study on the method of separation and purification in Nosema pernyi

Key Words：Antheraea pernyi, Nosema pernyi, separation and purification

Abstract：     Nosema pernyi (Wen et Ding) is a lethal pathogen caused pebrine disease in Antheraea pernyi (GuérinMéneville). Pebrine disease, which is vertically transmitted, is one of the most harmful diseases of A.pernyi. Better information on this disease and its vector are required to prevent outbreaks. This paper compares two methods of separating and purifying the spores of N.pernyi; differential centrifugation and Percoll density gradient centrifugation. The results show that discontinuous density gradient centrifugation was superior to single density gradient centrifugation. The purity of spores centrifuged at 15 000 r/min for 30 min under a discontinuous density gradient was 25%, 50%, 75% and 100%.