Issue:ISSN 2095-1353
CN 11-6020/Q
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2012年49 No.5
The expression of the mRNA and cloning for the vitellogenincDNA in the bumblebee，Bombus ignitus
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Key Words：bumblebee，Bombus ignitus，vitellogenin，quantitative real-time RT-PCR
Abstract： Bombus ignitus Smith is one of the important pollinators of many economically important crops and wildflowers．vitellogenin (Vg) plays a key role in the control of reproduction and reproductive behavior of this species． In this study，Vg cDNA of B． ignitus was cloned and sequenced． It is composed of 5 481 bp and contains an ORF of 1 772 amino acidswithin a putative signal peptide of 16 residues (GenBank accession number FJ913883) ． Close to the C-terminus there is aGL / ICG motif followed by nine cysteines，and a DGXR motif is located 18 residues upstream of the GL / ICG motif．BLASTX analysis of the conceptual translation products revealed 95% similarity to B． ignitus from Korea and to B．hypocrita． The deduced amino acid sequence shows significant similarity with Apis mellifera (51% ) and a high number ofconserved motifs． The mRNA of B． ignitus vitellogenin(Vg)was analysed in queens，workers and drones，from pupae toadults． Vg mRNA was found in the white-eyed pupal ( Pw) stage in queens and its expression increased during the entirepupal development，reaching a peak in the dark brown pupal ( Pbd) stage． It also had a high expression in the adult fatbody． In workers，the Vg expression was detected in the white-eyed pupal ( Pw) stage，its levels increasing with age andreaching a high point in 15 d，after which it decreased progressively． Vg mRNA was also observed in drones，the highestlevel of expression being observed in newly emerged adult drones．