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2012年49 No.5
Detection and identification of Nosema ceranae by dual fluorescent staining with Calcofluor White M2R and Sytox Green
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Key Words：Nosema ceranae，fluorescent staining，Calcofluor White M2R，Sytox Green
Abstract： Nosema ceranae is a microsporidian parasite of Apis cerana，Apis mellifera and bumblebees that has an adverse effect on pollination，especially of fruits and vegetables，and apiculture． In this study，Calcofluor White M2R and Sytox Green stains were used to discriminate between live and deadN． ceranae． The results show that deadN． ceranae spores had yellow-green fluorescence whereas live spores fluoresced white－blue． This method allows easy detection and identification of live and deadN． ceranae and should therefore contribute to further research onN． ceranae and itstreatment．