Rapid identification of aphids on Amygdalus plants using DNA barcoding
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Key Words：aphid, DNA barcoding, COⅠ, Amygdalus plant
Abstract： Amygdalus plants are important fruit trees and some are common ornamental plants. They are also the host plants for a variety of aphids. It is difficult to identify aphids rapidly and accurately by traditional methods because of the degradation of specimens, specialization of morphological characters and the occurrence of multiple forms within species. In order to identify aphids rapidly and accurately, DNA barcoding based on COI sequences was used to analyze aphids on Amygdalus plants. In this study, 12 species were found, including 10 species from Aphidinae, 1 species from Greenideinae and 1 species from Chaitophorinae. 96 COI sequences were obtained from this study. The average intraspecific divergence was 0.76% while interspecific divergence was 5.7%\|15.5%. NJ trees were built based on COI sequences, in which most samples of the same species clustered together with strong support (more than 95% bootstrap value). Combining sequence divergences and phylogenetic tree results, 99% of the aphid species on Amygdalus plants were well\|differentiated by DNA barcoding.