Research into the taxonomic status of three kinds of scarabs
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Key Words：carab, hybridization, COⅠ
Abstract： Holotrichia gebleri Faldermann was experimentally hybridized, with Holotrichia diomphalia Bates and Holotrichia oblita Faldermann. An F2 generation was then obtained from each F1 generation, demonstrating that males and females of each hybrid population were fertile. Mitochondrial cytochrome C oxidase I (COⅠ) gene fragments of three parent scarab species were cloned and sequenced and the genetic distance of these sequences analysed. These sequences were used to produce four kinds of phylogenetic trees. The results show that the genetic distance between the three species is similar and that each is distinct from H.parallela Motschulsky. We conclude that H. gebleri, H. diomphalia and H. oblita are three geographic populations of the same species.