Abstract:Leptocybe invasa Fisher & La Salle prefer laying eggs on new branches of Eucalyptus plants. The oviposition behavior of this
insect on E. urophylla × E. camaldulensis was investigated. Before comming in the laying cycle L. invasa must go through a
process of host discrimination and exploration. It can lay two eggs in one cycle, and running or walking while drumming
followed changing in oviposition sites. Based on a sample of 15 female wasps, the average number of eggs per female was 13
60 and the average duration for laying one egg was 1731 s. In the L. invasa laying egg process, the frequency of prying
behavior was significantly higher, and standing behavior was obviously lower, than other behaviors. On E. urophylla × E.
camaldulensis, L. invasa favored laying eggs on petioles and midribs, and there were no significant differences in the
number of eggs laid on these sites, but on E. urophylla, E. grandis ×E. urophylla, E. exserta, and E. propinqua, it
significantly favored petioles to midribs and twigs.