Abstract:Based on the original artificial diet formulation, an improved artificial diet for Helicoverpa assulta (Guenée) larvae was
developed by incorporating a proportion of chili powder as a feeding stimulant, and removing the tomato paste included in
the original diets. The effects of six artificial diets on biological parameters, nutritional indices and life table
parameters of H. assulta were investigated. The results indicate that there was no significant difference between an
artificial diet with 3% tomato paste and the control diet with respect to larval developmental period, pupa weight and
fecundity. However, larvae fed the artificial diet containing chili powder (from 05% to 4% wet weight) had significantly
higher survival rate, relative consumption rate and pupal weight than those on the control diet. The digestion of H. assulta
larvae was inhibited by the addition of ≥ 2% chili powder and adult mating ability significantly decreased when fed a diet
containing 4% chili powder. The optimum formulation was the control diet plus 1% chili powder. H. assulta fed on this diet
exhibited significantly higher fecundity and intrinsic rate of increase than those fed on other diets. The improved
artificial diet was suitable for rearing H. assulta in the laboratory and mass rearing for the commercial production of
nuclear polyhedrosis H. assulta viruses.