Issue:ISSN 2095-1353
CN 11-6020/Q
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2013年50 No.2
Cloning and bioinformatic analysis of the citrus red mite, Panonychus citri, prophenoloxidase activating enzyme gene
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Key Words:Panonychus citri, prophenoloxidase activating enzyme (PPAE), RACE, bioinformatic analysis
Abstract:Prophenoloxidase, a member of the serine proteinase group, is a key enzyme in invertebrates for defense against microbial infections and the prophenoloxidase activating enzyme (PPAE) is an important component of the prophenoloxidase activating system (PPOAS). A fulllength cDNA of the PPAE gene named PcPPAE (GenBank: KC136292) was cloned from Panonychus citri (McGregor) using rapid amplification of cDNA ends (RACE). The cloned gene was 1 676 bp in length with an ORF of 1 377 bp encoding 458 amino acid residues. The protein predicted from the cloned PcPPAE had a molecular weight of 49.9 ku and a theoretical isoeletric point (pI) of 5.68. Its predicted molecular formula was C2201H3467N607O678S22. Bioinformatic analysis showed that its instability index was 41.51 and its GRAVY was -0.268. The deduced protein PcPPAE had a clipdomain, serine proteinase domain and a conserved catalytic triad in the serine protease domain. Phylogenetic analysis indicates that PcPPAE is most closely related to the clipdomain serine proteinase of Tetranychus urticae (Koch). These results provide the basis for further research on defense against microbial infection in the citrus red mite.