Techniques used in insect neurobiological research: backfilling neurons with cobalt.
Author of the article:WANG Chen-Zhu
Author's Workplace:(State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China).
Key Words:staining neurons; backfilling with cobalt; locust
Abstract:Being able to see the structure of neurons is important in studying the nervous system of insects. Cobalt backfilling is a powerful tool for staining neurons. The procedure involves filling neurons with cobaltous salt, producing cobalt sulfide, then fi