The application of RNA extraction and realtime fluorescent quantitative PCR in insect

Author of the article:DONG XiaoLin

Author's Workplace：Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou510642, China

Key Words：insect，RNA extraction，integrality，realtime fluorescent quantitative PCR

Abstract：Using the brown planthopper as an example, we introduce a modified protocol of RNA extraction using the Trizol reagent method. Formaldehyde modified gel electrophoresis, NanoDrop (Thermo) and Agilent 2100 Bioanalyzer detection indicated that the RNA extracted by this method had high quality and perfect integrality. Several issues in the use of real\|time fluorescent quantitative PCR (RT\|qPCR) technique on insects became apparent in the expression pattern analysis. These results may provide a reference for the application of this technique in entomology.