Evolutionary relationships among the higher taxa of longicorn beetles (Coleoptera: Cerambycoidea) based on ribosomal 18S rDNA
Author of the article:WEI Zi-Han1** YIN Xin-Ming1*** AN Shi-Heng1 LI Jing1 ZHANG Hong-Fei2
Author's Workplace：1. College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China;2. Life Science of Henan Agricultural University, Zhengzhou 450002, China
Key Words： longicorn beetles, 18S rDNA, molecular marker, evolutionary relationships
Abstract： [Objectives] To reconstruct relationships among the higher taxa of the Cerambycoidea to provide a foundation for further phylogenic study of subfamilies in this taxon. [Methods] Phylogenetic relationships among 70 species representing 6 subfamilies and 3 families were investigated, including 49 newly sequenced species and 21 species for which gene sequences were obtained from the GenBank database. Partial ribosomal 18S rDNA (V4, V7) data were chosen to reconstruct phylogenic trees based on Neighbor Jointing (NJ), Maximum Likelihood (ML) and Bayesian Inference (BI) methods. [Results] Sequence analysis indicated that the 703 sites obtained after alignment contained 98 variable sites and 45 parsimony informative sites accounting for 13.9% and 6.4% of the total, respectively. The percentage of A, T, C, and G was 21.1%, 26.3%, 23.6% and 28.9%, respectively. The transition:transversion ratio reached 2.79. Phylogenetic trees based on Neighbor Jointing (NJ), Bayesian Inference (BI) and Maximum Likelihood (ML) methods were consistent with the traditional morphological classification in indicating that the Lamiinae, Cerambycinae, Prioninae, Disteniidae are monophyletic groups. [Conclusion] Phylogenetic trees can be successfully reconstructed for higher taxa of the Cerambycoidea and the ribosomal 18S rDNA is a useful genetic marker for this task.