[Objectives]  To assess the development of resistance, and cross-resistance, in Amphitetranychus viennensis Zacher to seven acaricides. [Methods]  Resistance was evaluated using biological assays conducted in a laboratory. [Results]  After selection for spirodiclofen resistance over 21 generations, the resistant strain had 11.65-fold resistance to that acaricide, 6.30-fold low level cross-resistance to etoxazole and 0.69-fold negative resistance to hexythiazox. Cross-resistance to abamectin, propargite and azocyclotin was 1.15, 1.25 and 1.78-fold, respectively, whereas resistance to pyridaben and bifenazate was 3.46 and 2.79-fold, respectively. [Conclusion]  A. viennensis has the capacity to develop resistance to spirodiclofen, and cross-resistance to some other acaricides. These results provide a theoretical basis for the rational rotation of pesticides in apple orchards.