Complete genome sequences and phylogenetics of the Lake Sinai virus in China
Author of the article:ZHANG Yi；DING Zhao-Run；LI Wen-Feng；HAN Ri-Chou
Author's Workplace：Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources 510260, China; South China Agricultural University, School of Agriculture, Guangzhou 510640, China
Key Words：Apis mellifera; Lake Sinai virus; phylogenetic analysis; recombination events
Abstract：
[Objectives] To obtain the
full-length genome sequence of the Lake Sinai virus (LSV) carried by Apis
mellifera in Guangdong Province, and investigate LSV polymorphism with
phylogenetic analysis. [Methods]
LSV GIABR was detected in
Guangdong, China in 2016. The full-length genome of this virus was sequenced
using Sanger sequencing and submitted to GenBank (accession number is
MT732482). Twenty-eight genome sequences and 34 RDRP sequences were downloaded
from GenBank, aligned and analyzed by MEGA.X using both the maximum likelihood
(ML) and neighbor-joining (NJ) methods. A recombination event was predicted by
RDP4.0. [Results] The complete genome sequence contained 5 910
nucleotides, with an ORF1 segment (genome position from 78 to 2615) overlapping
the RDRP segment (from 1852 to 3720), a capsid protein segment (from 3739 to
5301) and a hypothetical protein segment (from 5346 to 5798). Analysis of the
genome lineage, RDRP gene and recombination events suggest that LSV GIABR
belongs to SLV2
and is a putative, major, parental sequence of LSV2 isolates from the United
States and Australia. [Conclusion] LSV GIABR detected in A. mellifera in Guangdong,
China belongs to the LSV lineage 2, and is predicted to be a major
parental sequence of LSV2 isolates from the United States and Australia.