[Objectives]  To investigate the adaptability of honeybees, and to provide basic data for the healthy feeding and management of honeybees in greenhouses. [Methods]  Colonies in strawberry greenhouses were monitored by recording their weight and photographing combs over periods of 1, 15, 30 and 60 d. Spectrophotometry was used to detect antioxidant enzyme and detoxification enzyme activity in honeybees. [Results]  Hives in greenhouses for periods of 1, 15, 30 and 60 d had an average of 4, 2.625, 2.375 and 0.6 combs, respectively. Colony size also decreased significantly as with the duration of time in the greenhouse (P<0.05). Of the antioxidant enzymes monitored, superoxide dismutase (SOD) activity was significantly higher at 15 d, 30 d and 60 d than at 1 d (P<0.05) and the activity of all detoxifying enzymes except acetylcholinesterase (AchE) increased with the number of days hives were in the greenhouse and the activity of glutathione S-transferase (GST) enzyme was significantly higher at 30 d and 60 d than at 1 d (P<0.05). [Conclusion]  The greenhouse environment adversely affects the survival and reproduction of honeybees and activates their antioxidant and detoxification enzyme systems.