Spatiotemporal expression of CYP4G15 and CYP4L4 and their response to high and low temperature stress in Spodoptera frugiperda
Author of the article:SHANG Tun YANG Mao-Fa YU Xiao-Fei CHEN Yi-Chai TIAN Tai-An
Author's Workplace：Institute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou University, Guiyang 550025, China; College of Tobacco Science, Guizhou University, Guiyang 550025, China
Key Words：Spodoptera frugiperda; temperature stress; CYP4G15; CYP4L4; differential expression
Abstract：
[Objectives] To explore whether CYP4G15 and CYP4L4 are involved in the
response to high and low temperature stress in Spodoptera frugiperda. [Methods] The expression of CYP4G15 and CYP4L4 were detected by RT-PCR at 36 ℃ and 4 ℃ in different developmental
stages and tissues. [Results]
Expression of both genes was highest in young larvae. Expression of CYP4G15 was highest in the epidermis, whereas that of CYP4L4 was highest in the
foregut. The two genes were differentially expressed at high and low
temperatures. [Conclusion] CYP4G15 and CYP4L4 may be involved in the synthesis of various endogenous substances
in S. frugiperda, as well as metabolic processes under high and low
temperature stress.