[Objectives]  To determine the synergistic effect of combining three spray adjuvants; Qiangliyuan, Organosilicone and Huainongte, with imidacloprid, abamectin and matrine. [Methods]  An indoor toxicity bioassay and field trials were carried out using the leaf-dipping, pest-dipping and spray, methods. [Results]  The relative synergistic effect of the three spray adjuvants was as follows: Qiangliyuan> Organosilicone> Huainongte. Applying imidacloprid, abamectin and matrine for 48 h resulted in LC₅₀ values for Bemisia tabaci nymphs of 37.714 6, 1.806 2, and 10.563 2 mg/L, respectively. These LC₅₀ values were reduced to 13.018 8, 1.099 0, and 2.711 6 mg/L by the addition of Qiangliyuan, to 16.190 0, 1.271 9, and 3.1609 mg/L by the addition of Organosilicone and to 20.085 1, 1.324 7, and 3.760 3 mg/L by the addition of Huainongte. Field trials showed that the effectiveness of all three pesticides was significantly improved by the addition of the three spray adjuvants. The addition of Qiangliyuan and Organosilicone significantly increased the effectiveness of all three pesticides against B. tabaci, and the addition of Huainongte either slightly improved, or did not change, the effectiveness of each pesticide. The effectiveness of imidacloprid, abamectin, and matrine with added Qiangliyuan peaked three days after application, reaching 97.11%, 90.83% and 88.17%, respectively, despite the dosage of each pesticide being reduced by 20% by the addition of this adjuvant. Addition of all three adjuvants reduced the dosage of each pesticide by 40%. Nonetheless, the effectiveness of imidacloprid, abamectin and matrine seven days after application was 75.55%-80.76%, 71.84%-79.12% and 68.60%-75.74%. [Conclusion]  The effectiveness of imidacloprid, abamectin and matrine against B. tabaci can be significantly improved, and the dosage of these pesticides reduced by 20%-30%, by adding the adjuvants Qiangliyuan, Organosilicone and Huainongte.