Relative infectiveness of six Cordyceps sinensis-related fungal strains to Hepialus armoricanus larvae
Author of the article:XIE Fang CHEN Zhao-He ZHU Yu-Lan SU Qiang-Jun CHEN Chen ZHANG Yu
Author's Workplace:School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China
Key Words:Cordyceps sinensis-related fungus; Hepialus armoricanus larvae; Ophiocordyceps sinensis; infection
Abstract:
[Objectives] To study the relative infectiousness of six fungal strains isolated from
different parts of Cordyceps sinensis to Hepialus armoricanus larvae. C. sinensis-related fungi are defined as
fungi isolated from wild C. sinensis that can infect and kill H. armoricanus larvae and interact with O. sinensis. [Methods] Biological characteristics of 6 strains of
related fungi were described and their ITS sequences were aligned to identify
the species. The infectiveness of the six strains of related fungi to H. armoricanus larvae were tested
separately. Two related fungi were tested in a joint infection experiment with O. sinensis. [Results] The 6 strains of related fungi all affected the growth of O. sinensis: The inhibition rate of the
2F-2, FD, TB-1 and TB-4 strains to O.
sinensis was close to 100%, that of the 3-5-2 strain was 4.32% and that of
the MR-1 strain was 77.27%. All six strains had the ability to infect H. armoricanus larvae. The infection rate of the 3-5-2 strain was 50.0%, that
of the 2F-2 strain was 85.0% and that of the MR-1 strain was 73.3%. The
infection rate of FD strain was
70.0%; the infection rate of the TB-1 strain was 64.0% and the infection rate
of the TB-4 strain was 65.0%. The mortality rate of
joint infections was lower than that of single-strain infections. [Conclusion] The 6 strains of related fungi were all infectious to H. armoricanus larvae and can interact with O.
sinensis.